 Chattanooga Red Wolves SC | Nicholas Amponsah | D | Previously with Montego Bay United FC |
 Chattanooga Red Wolves SC | Steven Beattie | M | Previously with Cork City FC |
 Chattanooga Red Wolves SC | Jonathan Caparelli | D | Previously with FC Golden State Force |
 Chattanooga Red Wolves SC | Richard Dixon | D | Previously with OKC Energy FC |
 Chattanooga Red Wolves SC | Conor Doyle | F | Previously with Jacksonville Armada FC |
 Chattanooga Red Wolves SC | Colin Falvey | D | Previously with Ottawa Fury FC |
 Chattanooga Red Wolves SC | Leo Folla | D | Previously with Härnösands FF |
 Chattanooga Red Wolves SC | Greg Hartley | GK | Previously with Atherton Collieries A.F.C. |
 Chattanooga Red Wolves SC | Alex Mangels | GK | Previously with Portland Timbers 2 |
 Chattanooga Red Wolves SC | Juan Mare | F | Previously with Real Monarchs SLC |
 Chattanooga Red Wolves SC | Andrew Moullin | M | Previously with ATL UTD 2 |
 Chattanooga Red Wolves SC | Ualefi Rodrigues dos Reis | M | Previously with Barretos Esporte Clube |
 Chattanooga Red Wolves SC | Sito Seoane | F | Previously with UMF Grindavík |
 Chattanooga Red Wolves SC | Josue Soto | M | Previously with AC Oulu |
 Chattanooga Red Wolves SC | Tony Walls | D | Previously with Saint Louis FC |
 Chattanooga Red Wolves SC | Éamon Zayed | F | Previously with Charlotte Independence |
 Chattanooga Red Wolves SC | Vangjel Zguro | D | Previously with FK Kukesi |
 FC Tucson | Roy Abergil | F | Previously with Bnei Yehuda Tel Aviv FC |
 FC Tucson | Kyle Bjornethun | D | Previously with Toronto FC II |
 FC Tucson | Guillermo Delgado | F | Previously with Rio Grande Valley FC |
 FC Tucson | Karsten Hanlin | M | Previously with Colorado Springs Switchbacks FC |
 FC Tucson | Austin Ledbetter | D | Previously with Saint Louis FC |
 FC Tucson | Luis Martinez | M | Previously free agent |
 FC Tucson | Gilbert Oord | D | Previously with AVV Zeeburgia |
 FC Tucson | Moshe Perez | M | Previously with University of Denver |
 FC Tucson | Raheem Somersall | M | Previously with Florida Gulf Coast University |
 FC Tucson | Colin Stripling | M | Previously free agent |
 FC Tucson | José Carlos Terrón | D | Previously with Sociedad Deportiva Barreda Balompié |
 FC Tucson | Kyle Venter | D | Previously with Penn FC |
 FC Tucson | Kody Wakasa | D | Previously with Phoenix Rising FC |
 FC Tucson | Kienan Weekes | GK | Previously with University of Portland |
 FC Tucson | Andrew Wheeler-Omiunu | D | Previously with Atlanta United |
 Forward Madison FC | J.C. Banks | F | Previously with Jacksonville Armada FC |
 Forward Madison FC | Zaire Bartley | F | Previously with MFK Vyskov |
 Forward Madison FC | Brian Bement | F | Previously with Jacksonville Armada FC |
 Forward Madison FC | Brandon Eaton | M | Previously with the Richmond Kickers |
 Forward Madison FC | Paulo Júnior | F | Previously with Penn FC |
 Forward Madison FC | Eric Leonard | M | Previously with Nerang SC |
 Forward Madison FC | Jeff Michaud | M | Previously with The Miami FC 2 |
 Forward Madison FC | Vital Nizigiyimana | M | Previously free agent |
 Forward Madison FC | Josiel Núñez | M | Previously with CD Universitaro |
 Forward Madison FC | Carl Schneider | D | Previously with IFK Åmål |
 Forward Madison FC | Don Smart | M | Previously with The Miami FC 2 |
 Forward Madison FC | Brian Sylvestre | GK | Previously with LA Galaxy |
 Forward Madison FC | Danny Tenorio | M | Previously with Naples United FC |
 Forward Madison FC | Connor Tobin | D | Previously with North Carolina FC |
 Greenville Triumph SC | Christopher Bermudez | M | Previously with Pachuca |
 Greenville Triumph SC | Dominic Boland | D | Previously with Asheville City SC |
 Greenville Triumph SC | Paul Clowes | M | Previously with Egerton FC |
 Greenville Triumph SC | Carlos Gomez | M | Previously with Young Harris College |
 Greenville Triumph SC | Sami Guediri | M | Previously with SIMA Aguilas |
 Greenville Triumph SC | Dallas Jaye | GK | Previously with Phoenix Rising FC |
 Greenville Triumph SC | Jake Keegan | F | Previously with St. Patrick's Athletic |
 Greenville Triumph SC | Evan Lee | D | Previously with the Richmond Kickers |
 Greenville Triumph SC | Omar Mohamed | M | Previously free agent |
 Greenville Triumph SC | Tyler Polak | D | Previously with Saint Louis FC |
 Greenville Triumph SC | Kevin Politz | D | Previously with the New York Red Bulls II |
 Greenville Triumph SC | Cameron Saul | M | Previously with Lenoir-Rhyne University |
 Greenville Triumph SC | Cole Seiler | D | Previously with Sacramento Republic FC |
 Greenville Triumph SC | Aaron Walker | M | Previously with the Atlanta Silverbacks |
 Greenville Triumph SC | Travis Ward | F | Previously with the Philadelphia Fury |
 Lansing Ignite FC | Pato Botello Faz | F | Previoulsy with Saint Mary's University |
 Lansing Ignite FC | Kyle Carr | M | Previously with Lansing United |
 Lansing Ignite FC | Omar Castro | M | Previously with TSC Vahdet Braunschweig |
 Lansing Ignite FC | Kevin Coiffic | D | Previously with FC Miami City |
 Lansing Ignite FC | Brandon Fricke | D | Previously with the Des Moines Menace |
 Lansing Ignite FC | Xavier Gomez | M | Previously with Minnesota United FC |
 Lansing Ignite FC | Kyle Ihn | GK | Previously with Reno 1868 FC |
 Lansing Ignite FC | Lewis Jones | M | Previously with Lansing United |
 Lansing Ignite FC | Nathan Lewis | M | Previously with Indy Eleven |
 Lansing Ignite FC | Rafa Mentzingen | M | Previously with Lansing United |
 Lansing Ignite FC | Nick Moon | F | Previously with Lane United FC |
 Lansing Ignite FC | Tumi Moshobane | M | Previously with Olivet Nazarene University |
 Lansing Ignite FC | Steeve Saint-Duc | F | Previously with Los Angeles FC |
 Lansing Ignite FC | Christian Silva | M | Previously with Jacksonville Armada FC |
 Lansing Ignite FC | Grant Stoneman | D | Previously with Loyola University Chicago |
 Lansing Ignite FC | Rhys Williams | D | Previously with Real Monarchs SLC |
 North Texas SC | Carlos Avilez | GK | Previously free agent |
 North Texas SC | Bicou Bissainthe | M | Previously with Real Hope Football Academy |
 North Texas SC | Ronaldo Damus | F | Previously with FC Dallas U-19 Academy |
 North Texas SC | Brecc Evans | D | Previously with FC Dallas U-19 Academy |
 North Texas SC | Abdoul Gaffar Ibrahim | D | Loan from MFK Vyskov |
 North Texas SC | Alfusainey Jatta | M | Loan from MFK Vyskov |
 North Texas SC | Ricardo Pepi | F | Previously with FC Dallas U-17 Academy |
 North Texas SC | Arturo Rodriguez | M | Previously with FC Dallas Academy |
 North Texas SC | Oscar Romero | M | Previously with Pumas UNAM |
 Orlando City B | Jordan Bender | M | Previously with Orlando City Development Academy |
 Orlando City B | Luc Granitur | F | Previously with Orlando City Development Academy |
 Richmond Kickers | Amass Amankona | M | Previously with Indy Eleven |
 Richmond Kickers | Matt Bolduc | M | Re-signed |
 Richmond Kickers | Dennis Chin | F | Previously with Pittsburgh Riverhounds SC |
 Richmond Kickers | Akira Fitzgerald | GK | Previously with the Tampa Bay Rowdies |
 Richmond Kickers | Josh Hughes | M | Previously with Nashville SC |
 Richmond Kickers | Daniel Jackson | F | Previously with Devonport City |
 Richmond Kickers | Eli Lockaby | M | Previously with Virginia Commonwealth University |
 Richmond Kickers | Mutaya Mwape | M | Previously with the Charlotte Independence |
 Richmond Kickers | Scott Thomsen | D | Re-signed |
 Richmond Kickers | Braeden Troyer | D | Re-signed |
 South Georgia Tormenta FC | Conner Antley | D | Re-signed from USL League Two |
 South Georgia Tormenta FC | Jad Arslan | M | Re-signed from USL League Two |
 South Georgia Tormenta FC | Micah Bledsoe | GK | Previously with Nashville SC |
 South Georgia Tormenta FC | Lucas Coutinho | M | Previously with Lakeland Tropics |
 South Georgia Tormenta FC | Charlie Dennis | M | Re-signed from USL League Two |
 South Georgia Tormenta FC | Lars Eckenrode | D | Previously with Toronto FC II |
 South Georgia Tormenta FC | Ricardo Gomez | M | Previously with Myrtle Beach Mutiny |
 South Georgia Tormenta FC | Christopher Hellmann | F | Previously with the Des Moines Menace |
 South Georgia Tormenta FC | Pablo Jara | GK | Re-signed from USL League Two |
 South Georgia Tormenta FC | Michael Mecham | D | Re-signed from USL League Two |
 South Georgia Tormenta FC | Marco Micaletto | M | Re-signed from USL League Two |
 South Georgia Tormenta FC | Alex Morrell | F | Previously with the Tampa Bay Rowdies |
 South Georgia Tormenta FC | Michael O’Sullivan | D | Previously with Jacksonville Armada FC |
 South Georgia Tormenta FC | Dalton Pando | M | Previously with FC Tucson |
 South Georgia Tormenta FC | Peter Pearson | M | Previously with the Des Moines Menace |
 South Georgia Tormenta FC | Joshua Phelps | D | Re-signed from USL League Two |
 South Georgia Tormenta FC | Mikie Rowe | F | Re-signed from USL League Two |
 South Georgia Tormenta FC | Jerry Saint-Vil | D | Re-signed from USL League Two |
 South Georgia Tormenta FC | Mitch Thorn | GK | Previously with Logan Lightning FC |
 South Georgia Tormenta FC | Nil Vinyals | M | Re-signed from USL League Two |
 South Georgia Tormenta FC | Nick Wells | M | Re-signed from USL League Two |
 Toronto FC II | Robert Boskovic | D | Re-signed |
 Toronto FC II | Dante Campbell | M | Re-signed |
 Toronto FC II | Jordan Faria | F | Under Contract |
 Toronto FC II | Shaan Hundal | F | Under Contract |
 Toronto FC II | Jahkeele Marshall-Rutty | F | Previously with TFC Academy |
 Toronto FC II | Noble Okello | M | Under Contract |
 Toronto FC II | Jordan Perruzza | F | Previously with TFC Academy |
 Toronto FC II | Luca Petrasso | M | Under Contract |
 Toronto FC II | Rocco Romeo | D | Under Contract |
 Toronto FC II | Jacob Shaffelburg | F | Previously with TFC Academy |
 Toronto FC II | Matt Srbely | M | Re-signed |
 Toronto FC II | Gideon Waja | M | Under Contract |